Syllabus of lectures: 1. Introduction to microbiology, an overview of the systematics of microorganisms. Morphology, structure, reproduction, growth and metabolism of microorganisms. 2. Mechanisms of microbial pathogenicity, sources and spread of infection. Microbial genetics. 3. Significant bacterial diseases. 4. Basics of virology. Significant viral diseases. 5. Introduction to parasitology, basic terms in parasitology, parasite and host, route of transmission of agents, specificity and pathogenicity of parasites. 6. Introduction to protozoology, general characteristics, morphology and basic systematics. 7. Introduction to helminthology, general characteristics, morphology and basic systematics. 8. Introduction to arachnoentomology. Arachnida (characteristics and distribution of ticks and scabies). Insecta (characteristics and distribution of the most important agents – lice, fleas, flies). 9. Immune system – properties and structure (cells and organs). Characteristics and properties of the specified and key. 10. Characteristics of the mechanisms of non-specific immunity: Complement and phagocytosis. 11. Characteristics of the mechanisms of specific immunity: cellular and humoral immunity 12. Immunoprophylaxis: vaccines. 13. Characteristics of immunopathological reactions (hypersensitive and autoimmune mechanisms). Syllabus of practical exercises: 1. Safety rules in the lab. Sampling. Preparation of nutrient media. Isolation and cultivation of bacteria on nutrient media. Evaluation of bacterial growth on culture media. 2. Staining methods. Testing of metabolic activity of bacteria, basic methods of detection of bacterial antigens, methods of testing resistance of bacteria to antibiotics. 3. Diagnostics of important zoonotic pathogens. 4. Basics of virological diagnostics. 5. General terminology, collection of material for parasitological examination. 6. Basic parasitological diagnostic methods, and basic criteria for diagnosing parasitic bodies (pseudoparasitic bodies). 7. Diagnostic methods in parasitology (native, concentration). 8. Preparation of the native preparation. 9. Principles of work in the immunological laboratory. Collection of material for immunological examination. Determination of immunoglobulin concentration: Coagulation test with glutaraldehyde, Turbidimeter test with zinc sulfate 10. Basic serological investigation methods: agglutination, precipitation, immunoenzymatic and immunofluorescence tests. 11. Evaluation of cellular immunity: isolation of leukocytes. 12. Assessment of cellular immunity: assessment of phagocytosis. 13. Hypersensitivity testing (in vivo and in vitro tests). |