Lectures (2 hours) 1. Past, present and future. Pathogenesis of virus infection (virulence, pathogenicity, host-pathogen interaction, course of virus infection) 2. Antivirus immunity. Factors of host resistance. Cell receptors and cytokines involved in the fight against virus infection. Avoiding of host immunity. 3. Antivirotics. 4. Oncogenesis and oncogenic viruses. 5. Non-enveloped DNA viruses (adeno-, papiloma-, polyoma-, parvo- and circoviruses). 6. Enveloped DNA viruses (herpes-, asfar- and poxviruses). 7. Non-enveloped icosahedral RNA viruses (picorna-, calici-, astro-, reo- and birnaviruses). 8. Enveloped icosahedral RNA viruses (toga-, flavi-, borna- and arteriviruses). 9. Enveloped helical RNA viruses (ortomyxo-, paramyxo-, rhabdo-, corona-, arena- and bunyaviruses). 10. Food-borne viruses. 11. Arboviruses. 12. Causative agents of spongiform encephalopathies I. 13. Causative agents of spongiform encephalopathies II. Practical labs (2 hours) 1. Characterization of a virological laboratory. 2. Preparation and cultivation of cell cultures. Cell counting and viability assessment. 3. Sampling for virological examination. Transport and saving of samples. Preparation of inoculum for isolation of viruses. 4. Characterisation and division of methods for virological diagnosis. 5. Virus quantification: virus titration, plaque formation test. 6. Detection virus antigens and anti-virus antibodies employing erythrocytes (hemagglutination test, hemagglutination inhibition test). 7. Detection anti-virus antibodies employing cell lines (virus neutralization test). 8. Detection of virus antigens by immunofluorescence. 9. Detection of virus antigens by immunochemistry. 10. Detection of virus antigens by immunoenzymatic reaction. 11. Molecular methods in viral diagnostics – PCR, RT PCR, real-time PCR, in situ hybridisation. 12. Flow cytometry in virus diagnostics. 13. Credit. |