100 % active participation on practical lessons, 2 credit tests (Microbiology part, Immunology part).

Scheme of the lectures (2 hrs):

Immunology

1. Immune system – characteristic and structure.Organs and cells of the immune system.

2. Characteristic and properties of antigen and antibodies.

3. Immune response – mechanisms of non-specific innate immunity. The complement system. Phagocytosis.

4. Immune response – mechanisms of specific adaptive immunity. Antigen presentation. MHC molecules. Cytokines.

5. Acquired immunity: T and B cells.

6. Inflammation. Immune response to pathogens and cancer.

7. Mucosal immunity. Immunity of fetus and newborn animals.

8. Hypersensitivity reactions.

9. Vaccination.

Microbiology

10. Clasification and identification of microorganisms. Pathogenic proteinaceous molecules – prions.

11. Interaction between host and microorganisms. Mechanisms of microbial and viral pathogenicity.

Parasitology

12. Introduction to Parasitology, Fundamentals of Protoozoology.

13. Fundamentals of Helminthology and Arachnoenthomology.

Scheme of the lessons (2 hrs):

Immunology

1. Agglutination

a) Tube agglutination test

b) Hemagglutination

2. Precipitation

a) Radial immunodiffusion (RID)

c) Immunoelectrophoresis

3. Enzyme immunoassay (EIA)

a) ELISA (Enzyme-Linked Immunosorbent Assay)

b) Western blot

4. Immunofluorescence assays (IFA)

a) fluorescence microscopy

b) flow cytometry

5. Isolation of leukocytes from blood

a) by lysis of erythrocytes with distilled water

b) by cell- separating solution

6. Testing of phagocyte functions

a) Phagocytosis of particles (latex, yeast or bacteria)

b) INT test - oxidative burst test

7. Testing of hypersensitivity

a) Credit test

Microbiology

8. Differential diagnostics of important G- bacteria (representatives of the family Enterobacteriaceae: genera Eschrichia, Salmonella, Klebsiella, Proteus, Shigella, Yersinia).

a) Microscopical examination

b) Cultivation properties

c) Metabolic activity of bacteria

d) Serological examinations

9. Differential diagnostics of representatives of G+ cocci (genus Staphylococcus and Streptococcus).

a) Microscopical examination

b) Cultivation properties

c) Metabolic activity of bacteria

d) Serological examinations

10. Differential diagnostics of representatives of G+ rods (genus Listeria, Mycobacterium and Bacillus).

a) Microscopical examination

b) Cultivation properties

c) Metabolic activity of bacteria

d) Serological examinations

11. Principles of virological diagnostics.

a) Methods of viral cultivation.

b) Division and characterization of cell cultures, media and materials.

c) Cytophatic effect

d) Credit test

Parasitology

12. Diagnostics of representatives of Protozoa.

13. Diagnostics of representatives of Helminths and Arthropods.

Recommended literature:

1. Tortora G. J. et al.: Microbiology. An introduction. Pearson, Benjamin Cummings, 2005.

2. Markey, B. et al.: Clinical Veterinary Microbiology (Second edition), Mosby Elsevier, 2013.

3. Quinn, P. J. et al.: Veterinary Microbiology and Microbial Disease (2nd ed.), Wiley-Blackwell, 2013.

4. Scott McVey, D. et al.: Veterinary Microbiology (3rd ed.), Wiley-Blackwell, 2013.

5. Pistl J. et al.: Veterinary virology, UVMP Košice, 2014.

6. Pilipčinec E. et al.: Practical lessons from Microbiology. UVMP in Košice, 2016.

7. Tizard I.R.: Veterinary immunology Seventh edition. Elsevier, 2004.

8. Taylor, M. A. et al.: Veterinary Parasitology, BlackwellPublishing, (3rd ed.), 2007.

9. Letková V., et al.: Handbook For The Diagnosis of Parasites, Part 1, 2, 3, 1998.

Continuous assessment:

2 credit tests (Microbiology and Immunology)

Conditions for completion of course:

100 % active participation on practical lessons, 2 credit tests (Microbiology part, Immunology part).

Final assessment:

Test from Immunology and from Microbiology/Parasitology.

Final result is a summary of credit and results from both tests.