100% participation in practical exercises. One absence from practical training (documented by a doctor) can be excused by the teacher. Successful completion of the continuous study control (the student must obtain at least 51%) - a test in the 13th week of the semester with the value of 100 points and evaluated according to Article 20 point 4 of the Study Regulations of UVLF in Košice on the basics of microbiology, parasitology and immunology.

By completing the course, the student will gain comprehensive knowledge of microbiology, parasitology and immunology with an emphasis on understanding the basic concepts, definitions, interrelationships of organisms and the basics of their diagnostics. The graduate will use the acquired knowledge for professional activity in the Chamber of Veterinary Surgeons of the Slovak Republic as auxiliary staff for private veterinarians in laboratory practice, education, and scientific research.

Within the subject, students will deal with the systematics of microorganisms, mechanisms of microbial pathogenicity, important bacterial, viral or parasitic diseases and especially the possibilities of their diagnosis and correct laboratory practice in conditions where infectious material is processed.

Syllabus of lectures:

1. Introduction to microbiology, an overview of the systematics of microorganisms. Morphology, structure, reproduction, growth and metabolism of microorganisms.

2. Mechanisms of microbial pathogenicity, sources and spread of infection. Microbial genetics.

3. Significant bacterial diseases.

4. Basics of virology. Significant viral diseases.

5. Introduction to parasitology, basic terms in parasitology, parasite and host, route of transmission of agents, specificity and pathogenicity of parasites.

6. Introduction to protozoology, general characteristics, morphology and basic systematics.

7. Introduction to helminthology, general characteristics, morphology and basic systematics.

8. Introduction to arachnoentomology. Arachnida (characteristics and distribution of ticks and scabies). Insecta (characteristics and distribution of the most important agents – lice, fleas, flies).

9. Immune system – properties and structure (cells and organs). Characteristics and properties of the specified and key.

10. Characteristics of the mechanisms of non-specific immunity: Complement and phagocytosis.

11. Characteristics of the mechanisms of specific immunity: cellular and humoral immunity

12. Immunoprophylaxis: vaccines.

13. Characteristics of immunopathological reactions (hypersensitive and autoimmune mechanisms).

Syllabus of practical exercises:

1. Safety rules in the lab. Sampling. Preparation of nutrient media. Isolation and cultivation of bacteria on nutrient media. Evaluation of bacterial growth on culture media.

2. Staining methods. Testing of metabolic activity of bacteria, basic methods of detection of bacterial antigens, methods of testing resistance of bacteria to antibiotics.

3. Diagnostics of important zoonotic pathogens.

4. Basics of virological diagnostics.

5. General terminology, collection of material for parasitological examination.

6. Basic parasitological diagnostic methods, and basic criteria for diagnosing parasitic bodies (pseudoparasitic bodies).

7. Diagnostic methods in parasitology (native, concentration).

8. Preparation of the native preparation.

9. Principles of work in the immunological laboratory. Collection of material for immunological examination. Determination of immunoglobulin concentration: Coagulation test with glutaraldehyde, Turbidimeter test with zinc sulfate

10. Basic serological investigation methods: agglutination, precipitation, immunoenzymatic and immunofluorescence tests.

11. Evaluation of cellular immunity: isolation of leukocytes.

12. Assessment of cellular immunity: assessment of phagocytosis.

13. Hypersensitivity testing (in vivo and in vitro tests).

1. Pilipčinec E., Pistl J. et al.: Practical lessons from Microbiology, UVMP in Košice, 2016.

2. Tortora G. J., Funke B.R., Case CH.L. Microbiology. An introduction. Pearson, Benjamin Cummings, 2005

3. Markey, B. et al.: Clinical Veterinary Microbiology (Second edition), Mosby Elsevier. 2013.

4. Tkáčiková Ľ.: Veterinary immunology, UVMP in Košice, 2018.

Content prerequisite:

Anatomy, Basics of laboratory diagnostics, Physiology, Basics of pharmacology

Continuous assessment:

Credit test

Conditions for completion of course:

100% participation in practical exercises. One absence from practical training (documented by a doctor) can be excused by the teacher. Successful completion of the continuous study control (the student must obtain at least 51%) - a test in the 13th week of the semester with the value of 100 points and evaluated according to Article 20 point 4 of the Study Regulations of UVLF in Košice on the basics of microbiology, parasitology and immunology.

Final assessment:

Exam