Molecular parasitology

Study programme: parasitic diseases of animals external form of study
Teaching language:   english
Subject code: KaBioaFyziol/MPar ef/COS/22    Short: MPar ef/COS
  •  Credits: 5
  •  Completion method: Examination
Form, course-load and method od study:
Method of study: present
 
Prerequisites a following
Žiadne
 
Teachers
Lecturer:
Instructor:
Guarantor:
CONDITIONS FOR COMPLETION OF COURSE
In order to pass the course, the student must actively participate in lectures and practical teaching. The condition for passing the exam is the preparation and defense of a seminar paper on a topic specified by the guarantor (in the range A-E), which is the result of self-study. The exam is awarded based on the evaluation of acquired knowledge (in the range A-E).
Learning outcomes
The result of studying the subject Molecular Parasitology is the acquisition of knowledge in the molecular organization and characteristics of the genome and in the methods of parasite diagnosis.
Brief outline of the course
Molecular parasitology, its history and subject of study, DNA replication and proteosynthesis. differences in prokaryotes and eukaryotes, Regulation of gene expression, Hybridization of nucleic acids, Hybridization probes, DNA barcoding, Fundamentals of genomics. Molecular analysis of the genome. Gene therapy and its perspectives. Bioinformatics
Course syllabus
Molecular parasitology - history and subject of study, structure and properties of DNA, importance and use of molecular parasitology in science and research. DNA Replication and Proteosynthesis. differences in prokaryotes and eukaryotes Regulation of gene expression - Gene expression and mechanisms affecting gene expression, Nucleic acid hybridization - DNA profile construction, DNA denaturation and renaturation, Hybridization probes, DNA labeling, Molecular methods based on DNA hybridization, DNA chips. DNA cloning - Recombinant DNA technologies, DNA barcoding as a method developed for the purpose of species identification using short DNA sequences, its use and significance in the identification of plant and animal organisms, in proving food adulteration, in the differentiation of cryptic species, or species of medical and pharmaceutical importance. Fundamentals of genomics. Molecular analysis of the genome. Gene therapy and its perspectives. Bioinformatics and orientation in DNA and protein databases Laboratory diagnostic procedures (different variations of PCR, DGGE, MLST, ELFO, sequencing,...)
Recommended literature
Valenčáková A., Luptáková, L.: Molecular biology for pharmacists. I. Edition, Košice UVLF, 2020, 186 p. ISBN: 978-80-8077-687-7 VALENČÁKOVÁ, Alexandra - LUPTÁKOVÁ, Lenka - DANIŠOVÁ, Oľga - BÁLENT, Pavol. Methods of molecular biology - instructions for practical exercises for the pharmacy study program. 1st ed. Košice: UVLF, 2015. 79 p. ISBN 978-80-8077-475-2.
Conditions for completion of course
Continuous assessment:
After mastering the methods of molecular biology and informatics and after an oral consultation on the use of these methods in solving the doctoral student's scientific problem, the student is allowed to award credits.
Conditions for completion of course:
In order to pass the course, the student must actively participate in lectures and practical teaching. The condition for passing the exam is the preparation and defense of a seminar paper on a topic specified by the guarantor (in the range A-E), which is the result of self-study. The exam is awarded based on the evaluation of acquired knowledge (in the range A-E).
Final assessment:
The awarding of credits and the final evaluation is conditional on the submission and defense of the seminar work.
 
Evaluation of the course
Total number of evaluated students: 0
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Date of last modification: 29.03.2023
Approved by: Tutot Prof. MVDr. Alica Kočišová, PhD.
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