Flow cytometry and its application in the study of cells, their functions and products. Fluorescence microscopy in the detection of cellular, subcellular and molecular structures. Immunohistochemical methods of studying cells and tissues on paraffin and cryosections. Confocal microscopy in veterinary medicine. Specific cytochemical staining methods. Cell blocking techniques and their applications in veterinary medicine.

The graduate of the course will gain knowledge about the histological composition of cells, tissues, their functions and products. Through a combination of investigation methods, he will gain knowledge about changes in the course of disease processes related to the quantity of cells, their morphology, cytokine spectrum, he will know the ways of cell death, metabolic and molecular pathologies, transport of substances, expression of surface, intracellular and intracellular markers, as well as the representation of not only immunocompetent cells, but also mesenchymal or epithelial cells in tissues changed by inflammatory or tumor processes.

Flow cytometry and its application in the study of cells, their functions and products. Fluorescence microscopy in the detection of cellular, subcellular and molecular structures. Immunohistochemical methods of studying cells and tissues on paraffin and cryosections. Confocal microscopy in veterinary medicine. Specific cytochemical staining methods. Cell blocking techniques and their applications in veterinary medicine.

Sharkey L.C., Radin M.J., Seelig D.: Veterinary Cytology. Wiley Blackwell, 2021, ISBN: 978-1-119-12570-9 Meuten D.J.: Tumours in Domestic Animals. Fifth Ed., Wiley Blackwell, 2017, ISBN: 978-0-813-82179-5 Kiernan J.A. Histological and Histochemical Methods. Theory and Practise. 5th Ed., 2015, ISBN: 978 1907904 32 S Mondal S. K.: Manual of Histological Techniques. 1st Ed., Jaypee Brothers Medical Publishers, 2017, ISBN 9386261197 Macey M.G.: Flow Cytometry. Principes and Applications. Humana Press, 2007, ISBN 10: 1-59745-451-6 Watson J.W.: Introduction to Flow Cytometry,Cambridge University Press, 1991, ISBN: 0- 521-61199-7 Kubitscheck U.: Fluorescence Microscopy: From Principles to Biological Applications, Second Ed., Wiley, 2017, ISBN:9783527338375 Liu J., Tan J.: Confocal Microscopy. Morgan&Clavpool Publishers, 2016, ISBN: 9780750328487

Continuous assessment:

Completion of the subject is conditional on active participation in lectures and practical teaching.

Conditions for completion of course:

Flow cytometry and its application in the study of cells, their functions and products. Fluorescence microscopy in the detection of cellular, subcellular and molecular structures. Immunohistochemical methods of studying cells and tissues on paraffin and cryosections. Confocal microscopy in veterinary medicine. Specific cytochemical staining methods. Cell blocking techniques and their applications in veterinary medicine.

Final assessment:

The condition for obtaining the necessary credits for the student will be the submission of a seminar work in the range of 10-15 pages and its evaluation in the range of A-E.