Microbiology

Study programme: general veterinary medicine full-time form of study
Teaching language:   english
Subject code: KaMBaI/GVM-Mic 2/15    Short: GVM-Mic 2
  •  Credits: 8
  •  Completion method: Credit and Examination
  •   Lectures: 2 / Practice: 3
  •   Semester: winter semester
Form, course-load and method od study:
Form of study: Lecture / Practical
Course-load: Per week: 2 / 3   -   Per study period: 26 / 39 (recommended, in hours)
Method of study: prezenčná
 
Prerequisites a following
Prerequisites:      
Following:    
 
Teachers
Lecturer:
Instructor:
Examiner:
Guarantor:
CONDITIONS FOR COMPLETION OF COURSE
Conditions for Microbiology course are 100 % active participation on practical lessons and 2 credit tests (minimum is 5.5 points, maximum is 10 points). Exam consists from practical and written part. Written exam (test) has 3 parts (General microbiology, Special bacteriology and Virology) that cannot be written separately. The final mark of exam is a summary of results from credit tests, practical exam and results from written part of the test.
Final mark: credit (10-20 %), practical exam (11-20 %) and test (30-60 %).
If the teaching is moved to the online space due to specific situations, there may be some changes in the number or nature of questions through the MOODLE platform, but the basic condition is to reach at least 51%.
Learning outcomes
After completing the subject Microbiology, students will acquire theoretical and practical knowledge of special bacteriology and virology, with regard to taxonomic classification, pathogenic factors of infectious agents and parthenogenesis of infections. At the same time, they will gain an overview of the application of investigative methods such as microscopic, culture, biochemical, serological and molecular-biological examinations in the diagnosis of important animal pathogens as well as pathogens with zoonotic potential. Students will get a general overview of the characteristic biological properties of individual microorganisms.
Brief outline of the course
Special bacteriology and mycology, characterisation and description of pathogens of animals and pathogens with zoonotic potential, pathogenesis of diseases, taxonomy of bacteria.
Special virology, characterisation and description of viral pathogens of animals and pathogens with zoonotic potential, pathogenesis of diseases, taxonomy of viruses.
Prions, characterisation and description of prion pathogens of animals and pathogens with zoonotic potential, pathogenesis of diseases.
Laboratory diagnostic methods for detection of bacterial and viral pathogens.
Course syllabus
Syllabus of the lectures:
1. Gram-negative aerobic rods and cocci. The Pseudomonadaceae, Legionellaceae and Neisseriaceae family.
2. Important genera (Alcaligenes, Brucella, Bordetella, Francisella) of Gram-negative aerobic rods and cocci.
3. The Enterobacteriaceae family I (Genus Escherichia, Klebsiella, etc.).
4. The Enterobacteriaceae family II (Genus Salmonella, Proteus, etc.).
5. The Vibrionaceae and Pasteurellaceae.
6. The Spirochaetaceae and Leptospiraceae family. Important genera of aerobic vibroid Gram-negat. bacteria (Genus Campylobacter).
7. Aerobic Gram-positive cocci (Genus Staphylococcus, Streptococcus).
8. Aerobic Gram-positive rods (Genus Corynebacterium, Rhodococcus, Erysipelotix, Listeria, Mycobacterium, Nocardia).
9. Aerobic and anaerobic Gram-positive spore forming rods – genera Bacillus and Clostridium.
10. The Rickettsiaceae, Chlamydiaceae and Mycoplasmataceae.
11. Classification and reproduction of animal viruses. Interaction of viruses with cells. Prions and prion diseases.
12. Characterization and classification of the DNA viruses.
13. Characterization and classification of the RNA viruses with cubic and helical symmetry.
Syllabus of practical lessons:
1. Diagnostics of representatives of the genus Pseudomonas and Burkholderia
2. Diagnostics of Escherichia coli
3. Diagnostics of representatives of the genus Salmonella
4. Diagnostics of representatives of family Enterobacteriaceae
5. Diagnostics of bacterial agents of respiratory diseases of animals, representatives of the genera Pasteurella, Haemophilus, Actinobacillus
6. A. Diagnostics of representatives of the genus Brucella and Mycobacterium. 1st Credit test.
7. Diagnostics of representatives of the genus Staphylococcus
8. Diagnostics of representatives of the genus Streptococcus
9. Diagnostics of representatives of the family Bacillaceae
10. Diagnostics of representatives of genera Listeria and Rhodococcus
11. Diagnostics of bacterial pathogens – individual work
12. Principles of virological diagnostics. 2nd Credit test.
13. Reproduction of viruses in cell cultures. Evaluation of protocols. Granting credits
Recommended literature
1. Holoda E., Pistl J., Pilipčinec E.: Microbiology – General Microbiology, Study outlines, Dep. Microbiol. and Immunol., 2007.
2. Holoda E., Pistl J., Pilipčinec E.: Microbiology – Bacterial Genetics, Study outlines, Dep. Microbiol. and Immunol., 2008.
3. Pistl J., Holoda E., Pilipčinec E.: Microbiology – Special bacteriology, G-negative bacteria, Study outlines, Dep. Microbiol. and Immunol., 2007.
4. Pistl J., Holoda E., Pilipčinec E.: Microbiology – Special bacteriology, G-positive bacteria, Study outlines, Dep. Microbiol. and Immunol., 2007.
5. Pistl J. et al.: Veterinary virology, UVLP Košice, 2014.
6. Pilipčinec E., Pistl J. et al.: Practical lessons from Microbiology, UVMP in Košice, 2016.
7. Prescott L.M., Harley J.P., Klein D.A.: Microbiology. WCB Publish., Edit. K. Kane, WCB Communications Inc., 2005.
8. Tortora G. J., Funke B.R., Case CH.L. Microbiology. An introduction. Pearson, Benjamin Cummings, 2005.
9. MacLachlan N. J. and Dubovi E. J.: Fanner’s Veterinary virology, fourth edition. Elsevier Inc., 2011.
10. Markey, B., Leonard, F., Archambault, M., Cullinane, A., Maguire, D.: Clinical Veterinary Microbiology (Second edition), Mosby Elsevier. 2013
12. P. J. Quinn, B. K., Markey, F. C. Leonard, E. S. FitzPatrick, S. Fanning, P. J. Hartigan: Veterinary Microbiology and Microbial Disease (Second edition), Wiley-Blackwell, 2013.
13. D. Scott McVey, Melissa Kennedy and M. M. Chengappa (2013): Veterinary Microbiology (Third edition), Wiley-Blackwell, ISBN 978-1-118-65340-1.
14. Lectures from Microbiology and Immunology.
Conditions for completion of course
Content prerequisite:
Chemistry, Biology, Latin terminology
Continuous assessment:
100 % active participation on practical exercises from Microbiology, individual work, 2 credit tests - in 6th and 12th wek of the semester (min. 5.5 points, max. 10 points/per test).
Conditions for completion of course:
Conditions for Microbiology course are 100 % active participation on practical lessons and 2 credit tests (minimum is 5.5 points, maximum is 10 points). Exam consists from practical and written part. Written exam (test) has 3 parts (General microbiology, Special bacteriology and Virology) that cannot be written separately. The final mark of exam is a summary of results from credit tests, practical exam and results from written part of the test.
Final mark: credit (10-20 %), practical exam (11-20 %) and test (30-60 %).
If the teaching is moved to the online space due to specific situations, there may be some changes in the number or nature of questions through the MOODLE platform, but the basic condition is to reach at least 51%.
Final assessment:
Credit: gives 1/5 of the final result.
Examination: consists of two parts: practical and written part (General Microbiology, Special Bacteriology and Virology). Practical exam is a condition for written part (test).
Practical part - oral exam (11-20 %). Written test: each part (10-20 points), together 30-60 %.
Final evaluation (minimum 51 %): is sum of credit, practical exam and written exam (all 3 parts).
LANGUAGE, WHICH KNOWLEDGE IS NEEDED TO PASS THE COURSE
  english   
NOTES
Students can attend consultations every week on Thursdays between 2.00-3.00 p.m. We suggest to use Moodle platform with presentations from the lectures and practical lessons and also MS Teams for distance form of the study.
 
Evaluation of the course
Total number of evaluated students: 178
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3.9310.6724.7241.0116.852.81
 
Date of last modification: 30.11.2022
Approved by: Tutot Dr. h. c. Prof. MVDr. Jana Mojžišová, PhD.
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