participate in exercises within the desired range and write the test successfully

Students will be acquainted with collection of the most frequently used biological samples for laboratory analyses, as well with various factors, which might influence the analyses’ results. Another topic is the theme of reference/normal values and their interpretation related to the clinical status and selection of laboratory methods and procedures. The subject will provide the major haematological, biochemical and other abnormalities observed within laboratory diagnostics. Within the subject, the students should understand principles of homeostatic disturbances diagnosing and their importance for clinical practice.

Lectures:

1. Internal environment – composition and changes, clinical and laboratory diagnostics, laboratory analysis, quality control, reference intervals, clinical biochemistry, units of measure

2.Haematological examination – sample collection, methods, specimens, parameters in large animals

3.Laboratoty sample collection in exotic pets and poultry of clinical importance

4.Quality laboratory control in dairy industry – principles, organisation, methods, quality control on the farm, quality control in dairy plant (raw milk before the processing, during milk processing, final products), payment for milk, legislation of EU

5.Intra vitam and post mortem parasitological examination, diagnostic procedures in Parasitology, safety, specimen collection and processing, shipment, storing and staining, isolation of organisms, quantifying parasites

6.Detection of parasite antigens, molecular diagnostic, special tests in parasitology

7.The use of immunological methods for the determination humoral immunity (review).The use of a flow cytometer to assess cell-mediated immunity.

8.Serological methods for bacterial and viral antigens used in routine diagnostic approach

9.Molecular methods based on detection of nucleic acid in bacterial and viral pathogens

10.Dermatology – skin lesions and laboratory diagnostics of tumours

11.Pathology and diagnosis of the small animal diseases

12.Laboratory methods and tests used in small animal diseases diagnostics

13.Examination of body fluids in food animals, sample collection, storage, rapid tests

Practicals

1.Equipments, instruments used in laboratory diagnostics, visit of clinical biochemistry laboratory, collection of blood in large animals, blood processing, blood serum, plasma, storage of blood, serum or plasma

2.Basic haematological assays, haematological procedures, automated haematological analysis, blood film examination

3.Blood collection techniques and sampling for cytology, microbiology, parasitology in birds and exotic pets

4.Temperature of milk, test for inhibiting agents in milk, organoleptic tests, determination of sediment in milk, milk constituents, fat and protein content in milk, lactometer test, analysis of milk from cows with mastitis, microbiological examination of milk and milk products

5.Microscopic examination – native preparation, flotation method, Mcmaster method for parasite identification

6.Microscopic examination – diagnosis of blood parasites

7.The use of immunological methods for the determination humoral immunity The use of a flow cytometer to assess cell-mediated immunity

8.Overview of serological methods used in detection of bacterial and viral pathogens (agglutination, precipitation, indirect imunofluorescesnce test, immunoperoxidase monolayer assay), Haemagglutination test (HT), Haemagglutination inhibition test (HIT), ELISA test

9.Polymerase chain reaction (PCR), Electrophoresis

10.Demonstration of animal necropsy, sampling of tissue for histology, and addition examination – bacteriology, virology, biochemistry, haematology, toxicolocy, parasitology

11.Demonstration and practical training of cytology, flow cytometry methods, histology and immunohistochemistry

12.Specimen collection for laboratory diagnostics in small animals

13.Sample collection of urine, rumen content, faeces, milk for analysis in food animals, rapid tests

Kerr M.G.: Veterinary Laboratory Medicine, Blackwell Science, 2002

Stockham S.L. and Scott M.A.: Fundamentals of veterinary clinical medicine, Blackwell Publishing Comp., 2002

Meyer D.J. and Harvey J.W.: Veterinary Laboratory Medicine – Interpretation and Diagnosis. Saunders, 2004

Continuous assessment:

the form of questions in the exercises

Conditions for completion of course:

participate in exercises within the desired range and write the test successfully

Final assessment:

written test