Scheme of the lectures (2 hrs): Immunology 1. Immune system – characteristic and structure.Organs and cells of the immune system. 2. Characteristic and properties of antigen and antibodies. 3. Immune response – mechanisms of non-specific innate immunity. The complement system. Phagocytosis. 4. Immune response – mechanisms of specific adaptive immunity. Antigen presentation. MHC molecules. Cytokines. 5. Acquired immunity: T and B cells. 6. Inflammation. Immune response to pathogens and cancer. 7. Mucosal immunity. Immunity of fetus and newborn animals. 8. Hypersensitivity reactions. 9. Vaccination. Microbiology 10. Clasification and identification of microorganisms. Pathogenic proteinaceous molecules – prions. 11. Interaction between host and microorganisms. Mechanisms of microbial and viral pathogenicity. Parasitology 12. Introduction to Parasitology, Fundamentals of Protoozoology. 13. Fundamentals of Helminthology and Arachnoenthomology. Scheme of the lessons (2 hrs): Immunology 1. Agglutination a) Tube agglutination test b) Hemagglutination 2. Precipitation a) Radial immunodiffusion (RID) c) Immunoelectrophoresis 3. Enzyme immunoassay (EIA) a) ELISA (Enzyme-Linked Immunosorbent Assay) b) Western blot 4. Immunofluorescence assays (IFA) a) fluorescence microscopy b) flow cytometry 5. Isolation of leukocytes from blood a) by lysis of erythrocytes with distilled water b) by cell- separating solution 6. Testing of phagocyte functions a) Phagocytosis of particles (latex, yeast or bacteria) b) INT test - oxidative burst test 7. Testing of hypersensitivity a) Credit test Microbiology 8. Differential diagnostics of important G- bacteria (representatives of the family Enterobacteriaceae: genera Eschrichia, Salmonella, Klebsiella, Proteus, Shigella, Yersinia). a) Microscopical examination b) Cultivation properties c) Metabolic activity of bacteria d) Serological examinations 9. Differential diagnostics of representatives of G+ cocci (genus Staphylococcus and Streptococcus). a) Microscopical examination b) Cultivation properties c) Metabolic activity of bacteria d) Serological examinations 10. Differential diagnostics of representatives of G+ rods (genus Listeria, Mycobacterium and Bacillus). a) Microscopical examination b) Cultivation properties c) Metabolic activity of bacteria d) Serological examinations 11. Principles of virological diagnostics. a) Methods of viral cultivation. b) Division and characterization of cell cultures, media and materials. c) Cytophatic effect d) Credit test Parasitology 12. Diagnostics of representatives of Protozoa. 13. Diagnostics of representatives of Helminths and Arthropods. |