Continuous assessment (e.g. written test, individual work...): written test covering the subject matter of practicals. It is necessary to gain min 51% - A 91%; B 81%; C 71%; D 61%; E 51% Request for credit: it is necessary to pass the credit written test. Final assessment (e.g. exam, thesis...): Written examination of knowledge and skills within the curriculum of the subject. Number of credits 2 - within a rating scale A - E.

Laboratory diagnosis in veterinary practice in primary diagnostics is intended to enable them to conduct "minimum program", since all the important parameters can be investigated using rapid test. In the form of a "minimum program" can be performed blood tests, urine, feces, and skin exudates.

Brief outline of the course: 1. Organization of practical courses, Introduction to Laboratory diagnostics (MVDr. Lenka Luptáková, PhD.) 2. Ethics guide - Experimenting on animals; Proposed EU directive; Guidelines for Ethical Conduct in the Care and Use of Nonhuman Animals in Research (MVDr. Zuzana Hurníková, PhD.) 3. Cell Culture as in vitro biological models – part I (MVDr. Lenka Luptáková, PhD.) 4. Cell Culture as in vitro biological models – part II (MVDr. Lenka Luptáková, PhD.) 5. Alternative biotesting using invertebrates – Artemia franciscana (part I; MVDr. Michaela Špalková, PhD.) 6. Alternative biotesting using invertebrates – Artemia franciscana (part II; MVDr. Michaela Špalková, PhD.) 7. Caenorhabditis elegans – model organism (MVDr. Lenka Luptáková, PhD.) 8. Bird embryo as alternative animal model for experiments (doc. MVDr. Eva Petrovová, PhD.) 9. Risk assessment of chemicals in aquatic organisms ((MVDr. R. Sabo, PhD.) 10. Risk assessment of chemicals in bees (MVDr. R. Sabo, PhD.) 11. Molecular diagnostics and genomics of pathogenic microorganisms (bacteria and viruses; MVDr. Janka Koščová, PhD.) 12. Using of flow cytometer in immunology (MVDr. Dagmar Mudroňová. PhD.) 13. Credit test and credit

Brief outline of the course (course syllabus): 1. Organization of practical courses, Introduction to Laboratory diagnostics (MVDr. Lenka Luptáková, PhD.) 2. Ethics guide - Experimenting on animals; Proposed EU directive; Guidelines for Ethical Conduct in the Care and Use of Nonhuman Animals in Research (MVDr. Zuzana Hurníková, PhD.) 3. Cell Culture as in vitro biological models – part I (MVDr. Lenka Luptáková, PhD.) 4. Cell Culture as in vitro biological models – part II (MVDr. Lenka Luptáková, PhD.) 5. Alternative biotesting using invertebrates – Artemia franciscana (part I; MVDr. Michaela Špalková, PhD.) 6. Alternative biotesting using invertebrates – Artemia franciscana (part II; MVDr. Michaela Špalková, PhD.) 7. Caenorhabditis elegans – model organism (MVDr. Lenka Luptáková, PhD.) 8. Bird embryo as alternative animal model for experiments (doc. MVDr. Eva Petrovová, PhD.) 9. Risk assessment of chemicals in aquatic organisms ((MVDr. R. Sabo, PhD.) 10. Risk assessment of chemicals in bees (MVDr. R. Sabo, PhD.) 11. Molecular diagnostics and genomics of pathogenic microorganisms (bacteria and viruses; MVDr. Janka Koščová, PhD.) 12. Using of flow cytometer in immunology (MVDr. Dagmar Mudroňová. PhD.) 13. Credit test and credit

Kraft, W., Durr, U. M.: Klinická laboratórna diagnostika vo veterinárnej medicíne. H&H, Bratislava 2001 Kokinčáková, T., Bálent, P., Hrebenár, S.: Laboratórne metódy v biológii. USVZ, Prešov 2011

Conditions for completion of course:

Continuous assessment (e.g. written test, individual work...): written test covering the subject matter of practicals. It is necessary to gain min 51% - A 91%; B 81%; C 71%; D 61%; E 51% Request for credit: it is necessary to pass the credit written test. Final assessment (e.g. exam, thesis...): Written examination of knowledge and skills within the curriculum of the subject. Number of credits 2 - within a rating scale A - E.

Final assessment:

Written examination of knowledge and skills within the curriculum of the subject. Number of credits 2 - within a rating scale A - E.