Conditions for completing the course:

The condition for passing the course is:

1. active participation in lectures and exercises (1 absence without compensation)

2. acquisition of theoretical knowledge in the field of regenerative medicine (answering questions from the topics discussed)

3. acquisition of basic laboratory principles with cell cultures

4. successful acquisition of credit by fulfilling the following conditions: participation in exercises (1 absence without compensation, another absence replaced in the form of seminar work)

5. passing the exam - written form (getting at least 51%)

The goal of the course is to acquire basic knowledge in the field of regenerative medicine and its practical use in the treatment of damaged and non-functional tissues and organs. The student will gain practical laboratory skills in working with biological material, cell cultures and laboratory equipment. The student is able to plan, perform, evaluate and interpret the results of the experiment.

- Basic concepts and introduction to regenerative medicine

- Use of stem cells and cell lines in regenerative medicine

- Possible methods of isolation, cultivation, differentiation and cryopreservation of cells

- Experimental models and pharmacological approaches in regenerative medicine

- Ethical principles in cell research

LECTURES:

LECTURE 1. Principles of cell culture laboratory operations - safety, devices

LECTURE 2. Introduction to regenerative medicine - history, types of stem cells

LECTURE 3. Properties of cell culture - primary culture, cell line, cell strain

LECTURE 4. Sterile conditions in the cell culture laboratory and biological contamination

LECTURE 5. Basic principles of cell culture - environmental conditions, media and plastics

LECTURE 6. Cell division and aging - principles of cell passaging and counting

LECTURE 7. Adult mesenchymal stem cells isolated from bone marrow

LECTURE 8. Adult mesenchymal stem cells isolated from adipose tissue

LECTURE 9. Neonatal mesenchymal stem cells isolated from umbilical cord

LECTURE 10. Brain neurogenesis, neural progenitor cells, iPS cells

LECTURE 11. Freezing and cryogenic storage of cell culture - media, conditions

LECTURE 12. Study of stem cells in in vitro and in vivo conditions

LECTURE 13. Clinical use of stem cells in human and veterinary medicine

EXERCISES:

EXERCISE 1. Principles of using laminar boxes, sterile material and reagents

EXERCISE 2. Methods of isolation and sources of stem cells: embryonic, adult, neonatal and iPCs

EXERCISE 3. Introduction to the preparation of 2D and 3D cell cultures: adherents, scaffolds

EXERCISE 4. Elimination of biological contamination, use of antibiotics and antifungals

EXERCISE 5. Principles of culture protocols, proliferation (MTT test) and cell aging

EXERCISE 6. Monitoring of morphology, cell density, passaging and cell counting

EXERCISE 7. Protocols for isolation of MSCs from bone marrow, FC, multilineage differentiation

EXERCISE 8. Protocols for isolation of MSCs from adipose tissue, FC, multilineage differentiation

EXERCISE 9. Protocols for isolation of MSCs from umbilical cords, multilineage differentiation

EXERCISE 10. Protocols for isolation of neural progenitor cells, IHC

EXERCISE 11. Principles of cryopreservation, storage and thawing of cell culture

EXERCISE 12. The design of in vitro and in vivo experiments

EXERCISE 13. Selection of optimal cells for the regeneration of specific tissues

CIZKOVA, D., et al. Basics of Regenerative Medicine: Principles and applications, UVMP 2022

AL-RUBEAI, M. Animal cell Culture. Berlin : Springer , 2015. - 763 p.

LÓPEZ-LARREA, C., et al.; Stem Cell Transplantation. New York : Springer Science, 2012. - 342 p.

MILNER, R.; Astrocytes. New Jerey : HUMANA PRESS , 2012. - 575 p.

SUN, T.; Neural Stem Cells and Therapy. Croatia: InTech, 2012. – 440 p.

Conditions for completion of course:

Conditions for completing the course:

The condition for passing the course is:

1. active participation in lectures and exercises (1 absence without compensation)

2. acquisition of theoretical knowledge in the field of regenerative medicine (answering questions from the topics discussed)

3. acquisition of basic laboratory principles with cell cultures

4. successful acquisition of credit by fulfilling the following conditions: participation in exercises (1 absence without compensation, another absence replaced in the form of seminar work)

5. passing the exam - written form (getting at least 51%)

Final assessment:

1. successful acquisition of credit by fulfilling the following conditions: participation in exercises (1 absence without compensation, another absence replaced in the form of seminar work)

2. passing the exam - written form (getting at least 51%)