Credit (laboratory work, protocol) and exam (written and power point presentation of selected topic from Microbiology and Immunology).

Lectures:

1. Introduction to clinical microbiology, its role and objectives in veterinary microbiology. Characterization and classification of the most common pathogens focused to zoonoses.

2. Microbiology of the most common infections of the particular organ systems in carnivores (dogs and cats).

3. Microbiology of the most common infections of the particular organ systems in horses.

4. Microbiology of the most common infections of the particular organ systems in pigs.

5. Microbiology of the most common infections of the particular organ systems in ruminants (cattle, sheep, goat).

6. Antimicrobial agents, principles of antibiotic options in clinical practice. Antiviral agents and their use.

7. Introduction to Clinical Immunology, its role and objectives in veterinary immunology.

Characterization and classification of the most common disorders of the immune system.

8. Immunodeficiency states.

9. The pathophysiology of allergic diseases.

10. The pathophysiology of autoimmune diseases.

11. Diagnosis and therapy of allergic diseases.

12. Basics of transplantation immunology, pathophysiology of HvG and GvH reactions.

13. Principles of immunomodulatory therapy.

Practicals:

1. The sampling methods for the microbiological examination of various animal species. Transport and storage of samples. Sampling kits.

2. Microbiological examination of individual organ systems in carnivores (dogs and cats).

3. Microbiological examination of individual organ systems in horses.

4. Microbiological examination of individual organ systems in pigs.

5. Microbiological examination of individual organ systems in ruminants (cattle, sheep, goat).

6. Evaluation of the effects of antimicrobial agents, principles of antibiotic options in clinical practice. Minimal inhibitory concentrations of antibiotics.

7. Sampling methods for immunoassays from various animal species. Transport and storage of samples. Sampling sets.

8. Hematological assessment: Determination of the total leukocyte count and differential white blood cell counts in peripheral blood.

9. Approximative determination of the concentration of immunoglobulins: using glutaraldehyde coagulation test and the zinc sulphate test.

10. Determination of leukocyte functions: determination of phagocytic activity and oxidative burst activity.

11. – 13. Elaboration and submission of protocols from microbiological and immunological diagnostics.

Questions for exam 1. Characteristics of the causal agents, therapy and prevention of organ infections in dogs and cats. 2. Characteristics of the causal agents, therapy and prevention of organ infections in horses. 3. Characteristics of the causal agents, therapy and prevention of organ infections in ruminants (cattle, sheep and goats). 4. Characteristics of the causal agents, therapy and prevention of organ infections in pigs. 5. Characteristics of the causal agents, therapy and prevention of organ infections in poultry. 6. Characteristics and classification of the most often disorders of immune system. 7. Primary and secondary immunodeficiences of ruminants, pigs and horses. 8. Primary and secondary immunodeficiences in dogs and cats. 9. Systemic and organ diseases caused by immune reactions. 10. Hypersensitive reactions in animals. 11. Autoimmune diseases. 12. Immunomodulation and immunosupresion.

Markey B. et al.: Clinical Veterinary Microbiology, Mosby Elsevier, 2013.

Quinn P. J. et al.: Veterinary Microbiology and Microbial Disease, Wiley-Blackwell, 2013.

Pistl J. et al.: Veterinary Virology. Košice, 2014.

Tkáčiková L.: Veterinary immunology. UVLF, 2018.

Tizard I.R.: Veterinary immunology. 10th Edition, 2018

Content prerequisite:

Biology, Histology and embryology, Biochemistry, Genetics, Physiology, Anatomy I, Anatomy II, Nutrition and feeding of animals, Microbiology, Immunology, Propedeutics, Pathological anatomy.

Continuous assessment:

100 % participation on laboratory lessons, protocol from microbiology and immunology.

Conditions for completion of course:

Credit (laboratory work, protocol) and exam (written and power point presentation of selected topic from Microbiology and Immunology).

Final assessment:

Credit and exam from Microbiology and Immunology, based on presentation of selected topics, evaluation is a summary of the results from both parts.