In order to pass the course, the student must have active participation in lectures and practical training. The prerequisite for passing the examination is the preparation and defence of a seminar paper on the topic assigned by the supervisor (in the range A-E), which is the result of self-study. The examination is awarded on the basis of an assessment of the knowledge acquired (in the A-E range).

The graduate of the compulsory elective course molecular biology will gain additional knowledge about PCR and its variations, primer design, analysis of gene expression data, analysis of data obtained from transcriptomic and proteomic analyses, immunogenetics and microscopy.

Advanced PCR methods, construction of genes and plasmids, preparation of expression cassettes, OE-PCR, qRT-PCR, gene and protein expression. Methods and processing of results. Transcriptomics and proteomics in immunology. Recombination techniques, gain-in mutants, deletion mutants, use of natural mutations in immunology. Random and directed mutagenesis and its use in immunology. High-throughput techniques for immunogenetics, e.g. NGS (Next generation sequencing). Imaging technologies and their use in immunology and microbiology.

Carson S., Miller H.B, Srougi M.C., Witherow D.S.: Molecular Biology Techniques: A Classroom Laboratory Manual, 2019, ISBN 978-0-12-818024-2

Shaughnessy M.F., Varela M., Varela A.F.: The World of Molecular Biology, 2021, ISBN 9781536193121

Kumar A., Shera S.S. : Basic Concepts of Molecular Biology, 2020, ISBN 978-81-942926-3-0

Kappelmann-Fenzl M.: Next Generation Sequencing and Data Analysis, 2021, ISBN 978-3-030-62489-7

Biassoni R., Raso A.: Quantitative Real-Time PCR: Methods and Protocols, 2019, ISBN 9781493998326

Conditions for completion of course:

In order to pass the course, the student must have active participation in lectures and practical training. The prerequisite for passing the examination is the preparation and defence of a seminar paper on the topic assigned by the supervisor (in the range A-E), which is the result of self-study. The examination is awarded on the basis of an assessment of the knowledge acquired (in the A-E range).