Attending lectures and practical exercises, active participation in practical exercises.

Demonstration of the required skills and theoretical knowledge, which are necessary for the subject. On the practical exercises, you need to bring work clothes and shoes, as we work with animals.

Written elaboration of a literature review on embryo transfer in selected animal species.

Successful passing of the final exam.

After completing the course, the student acquires practical skills in obtaining oocytes and embryos.

Application of the superovulatory reaction of animals after the application of gonadotropic hormones. Knowledge mechanisms of oocyte maturation and morphological-functional states of oocytes and early embryos.

After completing the subject, the student will acquire manual skill in handling oocytes

and embryos in laboratory conditions. The acquired knowledge will create prerequisites for his

successful application in commercial or experimental practice implementing biotechnical or

biotechnological methods.

Course contents:

- History and importance of assisted reproduction for veterinary medicine and for commercial use.

- Embryo transfer in individual species of animals (cow, mare, pig, small ruminants, laboratory and wild animals). Knowledge of material and technical security, methodological procedures.

- Micromanipulations with gametes and early embryos (oocyte aspirations, in vitro fertilization,

cryopreservation of zygotes, bisection, sexation, cloning, production of transgenic individuals).

- Ethical and veterinary health conditions of handling gametes and early embryos.

Lectures:

1. History and Importance of assisted reproduction for veterinary medicine and for commercial use

2. Embryo transfer in cattle (preparation of donors and receipt - selection of animals, superovulation, synchronization)

3. Embryo transfer in cattle (material and technical equipment- apparatus for embryo collection, mediums)

4. Embryo transfer in cattle (collection and transfer of embryos)

5. Embryo transfer in mares

6. Embryo transfer in pigs

7. Embryo transfer in sheep and goat

8. Embryo transfer in laboratory and wild animals

9. Cryopreservation of embryos and oocytes

10. Micromanipulation of gametes and early embryos (bisection, sex determination, production of transgenic animals)

11. In vitro fertilization of oocytes

12. Cloning embryos

13. Ethical and health conditions manipulation with gametes and embryos

Practicals:

1. Embryo transfer in cattle (preparation of donors and recipients - selection of animals, superovulation, synchronization)

2.- 3. Embryo transfer in cattle - nonsurgical embryo collection

4. Preparation of embryos for embryo transfer (classification, cultivation, evidence)

5. - 6. Transvaginal oocyte aspiration in cattle

7.-8. Embryo transfer in mares - nonsurgical embryo collection

9.-10. Surgical collection of embryos in farm animals (sheep, sows)

11. Cryopreservation of embryos

12. Micromanipulation of gametes and early embryos (bisection, microinjection)

13. In vitro fertilization of oocytes

Singh,B., Gautam, S.K., Chauhan, M.S.: Textbook of animal biotechnology, The Energy and

Resources Institute, TERI, 2013, 620s., ISBN: 9788179933275

Content prerequisite:

Anatomy I.“ and „Anatomy II.“ and „Biology“ and „Physiology“ and „Histology and embryology“ and „Pharmaceutics and therapeutics“ and „General reproduction and obstetrics“ and „Andrology and artificial insemination“ and „Surgery“.

Continuous assessment:

individual work within practicals

Conditions for completion of course:

Attending lectures and practical exercises, active participation in practical exercises.

Demonstration of the required skills and theoretical knowledge, which are necessary for the subject. On the practical exercises, you need to bring work clothes and shoes, as we work with animals.

Written elaboration of a literature review on embryo transfer in selected animal species.

Successful passing of the final exam.

Final assessment:

Successful passing of the final exam.